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01July/2012 最近の、DAG研究の動向と方向性 基本的に、あまり進んでいない。2006年のうちらの論文および2010年のNewton ACの論文によるそれぞれの膜においてのシグナルのcompartmentalizeが、cell biologyのDAGに関する新しい発見である。これは、imaging技術の発展なしにしてはわからなかったことである。 24/Mar/2011 I found this paper with the search with the word, SMase and diacylglycerol. Methylmercury-induced toxicity is mediated by enhanced intracellular calcium through activation of phosphatidylcholine-specific phospholipase C D609 06/Dec/2010 These are the papers related to that DAG is cruical for membrane funsion. 1. Biochemistry. 1989 May 2;28(9) 3703-9. Physiological levels of diacylglycerols in phospholipid membranes induce membrane fusion and stabilize inverted phases. Siegel DP, Banschbach J, Alford D, Ellens H, Lis LJ, Quinn PJ, Yeagle PL, Bentz J. Procter and Gamble Company, Miami Valley Laboratories, Cincinnati, Ohio 45239-8707. 2.J Cell Biol. 2004 Dec 20;167(6) 1087-98. Interdependent assembly of specific regulatory lipids and membrane fusion proteins into the vertex ring domain of docked vacuoles. Fratti RA, Jun Y, Merz AJ, Margolis N, Wickner W. Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA. In this paper, they say DAG has inhibitory effect on vesicle fusion. 3.J Biol Chem. 2004 Dec 17;279(51) 53186-95. Epub 2004 Oct 12. Diacylglycerol and its formation by phospholipase C regulate Rab- and SNARE-dependent yeast vacuole fusion. Jun Y, Fratti RA, Wickner W. Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755-3844, USA. Paper In this paper, they say that DAG is masked by C1B domain, vacuola fusion is inhibited. 4. Biochem Biophys Res Commun. 2010 Apr 9;394(3) 733-6. Epub 2010 Mar 15. A novel complete reconstitution system for membrane integration of the simplest membrane protein. Nishiyama K, Maeda M, Abe M, Kanamori T, Shimamoto K, Kusumoto S, Ueda T, Tokuda H. Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan. nishiyam@iwate-u.ac.jp paper 5.Biochim Biophys Acta. 2010 Jan;1798(1) 59-64. Epub 2009 Nov 3. End-products diacylglycerol and ceramide modulate membrane fusion induced by a phospholipase C/sphingomyelinase from Pseudomonas aeruginosa. Ibarguren M, Bomans PH, Frederik PM, Stonehouse M, Vasil AI, Vasil ML, Alonso A, Goñi FM. Unidad de Biofísica (Centro Mixto CSIS-UPV/EHU), y Departamento de Bioquímica, Universidad del País Vasco, Apto. 644, 48080 Bilbao, Spain. Paper 6.Biophys J. 2009 May 6;96(9) 3638-47. A comparison of the membrane binding properties of C1B domains of PKCgamma, PKCdelta, and PKCepsilon. Sánchez-Bautista S, Corbalán-García S, Pérez-Lara A, Gómez-Fernández JC. Departamento de Bioquímica y Biología Molecular A, Facultad de Veterinaria, Universidad de Murcia, E-30080-Murcia, Spain. Paper 7.Biochim Biophys Acta. 2009 Mar;1788(3) 701-7. Epub 2008 Dec 3. Calcium inhibits diacylglycerol uptake by serum albumin. Ahyayauch H, Arana G, Sot J, Alonso A, Goñi FM. Unidad de Biofísica (Centro Mixto CSIC-UPV/EHU), and Departamento de Bioquímica, Universidad del País Vasco, Aptdo. 644, 48080 Bilbao, Spain. Paper 8. Progress in Lipid Research Volume 38, Issue 1, January 1999, Pages 1-48 Structure and functional properties of diacylglycerols in membranes Félix M. Goñi* and Alicia Alonso Grupo Biomembranas (Unidad Asociada al CSIC), Departamento de Bioquímica, Universidad del País Vasco, Aptdo. 644, 48080 Bilbao, Spain Paper 20Dec2010 About the experiment for biological significance of outer DAG. Previously I did experiment about the difference in cell growth between the cells with or without DAG probe. At the time, it seems that the flowting cells exist more in the cells incubated with DAG probe. This means that cell growth is suppressed with DAG probe. But MDCK cells s growth is very fast. I am gonna reduce the % of serum and make growth rate slow down. 14Dec2010 I need to finish this paper now around. But there are several things I have to do... 1. see if DAG comes out to the outer plasma membrane in response to ATP. 2. biological significance of outer DAG. The day after tomorrow, I gonna do the following expreiments. 1. Time course of outer DAG production in response to ATP in MDCK cells. 2. inhibitor of R59949 and U73122 at the time point of 10 min 3. mGluR expressed MDCK cells 1. SMnaseBcPLC, SMnase, ATP, ctrl. 1. ctrl(no D609),ctrl(D609), 1min(D609,ATP) 4min, 7min,10min. 2. ctrl(no D609), ctrl(D609), ctrl(D609ATP)D609ATPU73122, D609ATPR59949, biological significance 1. cell growth 2. incorporation of LDL or something. 24/Nov/3010 I am gonna do the following experiments. 1) 1. Incubate with 1 microM ATP and 10 U SMase and 12.5 unit/ml Bc-PLC for 4 min and 10 min and 10 min respectively. Do nothing about ctrl. 2. Fix cells with 3 % PFA for 15 min. 3. Add NH4Cl for 5 min. 2) Preincubation with D609 for 5 hour. 1. Preincubation of the cells with 15micro/L U73122 for 10 min shortly before starting experiments. 2. ATP stimulation and after 10 min, incubate with EFYP-C1AB for 2 min. 3. Fix cells with 3 % PFA for 15 min. 4. Add NH4Cl for 5 min. 22/Nov/2010 Transfection of DsRED2 and EYFP-C1AB into MDCK cells. What I want to do is that examination of DAG in stady state in ctrl SMase, Bc-PLC and ATP treated MDCK cell. 28/Oct/2010 I am gonna do the experiment for the paper. 1. incubation with D609 for 0, 1, 3, 6 and 12 hrs. 2. ATP stimulation after incubation with D609 for 6 hrs. 3. the effect of SMase, ATP and BcPLC on the inner DAG. 4. the comparison between Bc-PLC and ctrl. 12 33 12 hour incubation finish 17 06 6 hour incubation finish 12 12 1 hour incubation finish 14 12 3 hour incubation finish. 15/Oct/2010 The things I am gonna do from now is as follows. 1. At first, it is very weird that DAG flip should occured in stady state upon addition of SMase or incubation with ISP1. So,I am gonna observe DAG in inner leaflet of plasma membrane. RFP and YFP-C1AB are expressed in MDCK cells. Fix this cell. If there are some making a difference in fluorescence between RFP and YFP, YFP-C1AB can detect inner DAG. After identifing this, I am gonna do SMase addition to MDCK cells. I gonna compare the fluorescence pattern with or without SMase. 14/Oct/2010 Today I am gonna do the experiment of staining of lymphocyte with DAG probe. The way to do is something like that. MDCK, SMS-+ and Jurkat cells. (Actually I picked up 5×105 lympho cells 50microl) I want to difference in staing between MDCK and other lympho cells. 1. trypsinize 2. rip off the cells. 3. spin down 4. replace medium with HBSS. 5. incubate with DG probe for 2 min 6. fixed with 3%PFA And then, I want to see the staining pattern of Jurkat cells which have aggrigation. 1. pich up several Jurkat cells on the glass base dish. 2. replace the medium with HBSS carefully in the glass base dish. 3. 2 min incubation with DG probe 4. wash with HBSS 3 times carefully 5. fixed with 3% PFA. 07/Oct/2010 今日のDG I examined the effect of D609 on DAG on the outer leaflet of plasma membrane several days ago. Previously I thought that DAG is gonna dissapear after 6hour. However I found out that DAG dissapear more quickly within 3 hour. Basically within 1 hour, almost of DAG dissapears.So I am gonna do the following experiment next. 1. Incubate with D609 for 3 hour. At this time, I believe that internalization of recepters does not occur. So I can examine DAG flop that is drived from DAG producing in response to ATP stimulation. 2. 100microM ATP stimulation and stain the outer leaflet of DAG at 0,2,4,7,10 min. 17/Sep/2010 I did following experiments. MDCK cells were incubated with 50microg/ml D609 for 6 hour. ripped off the cells with trypsine. spread MDCK cells 24 well dish with D609. After 1 hrs, wash out the flouting cells 3 times, fixed and counted the number of cells. In control cells without D609, cells attached the bottom of dish well. However in the presence of D609, cells hardly attached the dish. 07/Sep/2010 The experiments below have been very much done. So what is the next step? The paper s theme is something involved in DAG flip-flop. So it is better focusing on DAG flip-flop. What I gonna do is as follow. 1. I am gonna check that there is no difference in cytosolic DAG production in response to ATP between with or without D609. I afraid of Receptors internalization during incubation with D609 as previously shown in some papers. If I can, I want to fix these cells because I can count cell numbers easily. 25/Aug/2010 MDCK was incubated with some drug as follows. 1. 10% LPDS 24 hrs 2. ISP-1 50 nM 24 hrs 3. FB1 40 microM 24 hrs 4. D609 50 mircog/ml 24 hrs 5. U73122 500 nM 24 hrs. 6. D609 50 microg/ml 24 hrs and U73122 500 nM 24 hrs. 7. D609 50 microg/ml 6 hrs 8. ctrl 19/Aug/2010 MDCK cells were incubated with 200 microM D609 for 12hour. But cells died perfectly. So I can not use long incubation with 200 microM D609. I am gonna try 6hour. 18/Aug/2010 The results of yesterday experiment. In fact, ISP-1 treated cells give annexin V fluorescence a little bit without Bc-PLC. After incubation with 1.25units/ml Bc-PLC, in the case of 200 nM ISP-1, fluoresecence of annexin V increases little by little. Same situation gonna happen in the case of 50 nM ISP-1. But labelling with annexin V is very weaker than 200 nM. This is the level I can ignore. So I put 50nM IPS-1 treated cells in my paper. 17/Aug/2010 Today I gonna do the following experiments. 13/Aut/2010, I did 12.5 unit/ml BcPLC application experiment. I want to confirm that ISP-1 treated cells do not have cell damages during incubation with 12.5 unit/ml Bc-PLC using anexin V. And I want to confirm whethere DAG produced by Bc-PLC is same between tISP-1 treated cells and normal cells. 13/Aug/2010 I did several important expriments. 1. whether ZS2, ZS2/SMS1 and ZS2/SMS2 have DAG on the outer leaflet of PM. Answer Every cells was stained with DAG probe at the same level. This shows that PC-PLC activity is different from SMS1 and SMS2. 2. whether D609 diminishes the outer DAG amount in MDCK cells. Answer at 6hour DAG diminished however 24 hour incubation recovered the DAG? What happen?? Anyhow I am gonna take reproducibility. 3. Glutamte applies MDCK cells expressing mGluR5. 20 microM glutamate was added to this MDCK cells. However there is no difference between not expressed cells and expressed cells. This data shows that DAG does not flop to the outer leaflet of the PM. 4. 1.25 unit/ml Bc-PLC was added to the MDCK cells treated with 50 and 200 nM ISP-1. Flip occured! 10/Aug/2010 Here is most important paper now for me. D609 D609-1 D609-2 In the experiment of 10/Aug/2010, we could not see difference in fluorescence between D609 24hour incubated MDCK cells and intact MDCK cell. So I am going to do this reproducibility again. And then I am gonna start ISP1 incubation again. 06/Aug/2010 I am gonna do ISP-1 treatment expeiment. I am gonna divide MDCK cells, which exposed 200nM and 50nM ISP-1 for observation and biochemistry expeiments. So I gonna prepare six 3.5mm well dishs for each. And I gonna do the experiment in which I want to see the effect of D609 on the receptor signalling. Because it is known that D609 causes the internalization of receptors from plasma membrane. So I prepare MDCK cells expressing inner DAG probe in three dishes. 03/Aug/2010 I got mGluR-RFP from Matsuura-kun. I can express this DNA whatever cells I want. I am gonna express this DNA CHO cells and MDCK cells. Today s result. I looked through the samples I prepared yesterday. Now I found out that DAG exists in static state. Based on this result, I need to plan the expriments. Basically, this expreiment follows the experiment of CHO cells. 02/Aug/2010 I am gonna do the experiment as follows. 1. no probe, probe only, ATP, ATP+R59949, ATP+U73122, PC-PLC. 2. MDCK cell growth with or without DAG probe. 01/Aug/2010 I am doing the experiments in which 10 microM R59949 incubation is done upon 100 microM ATP addition. I expect that more DAG comes to outer leaflet of PM than without R59949. 10 min R59949 incubation and then ATP stimulation containing R59949. 2 min incubation with DAG probe.
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インペリアル・ドラゴン Dragon, Imperial 出典 Bestiary 3 74ページ Imperial dragons, namesake of the Dragon Empires and guardians of Tian Xia before humanity arrived, embody five strengthening and counteracting forces. Unlike other true dragons, imperial dragons dive deep into human affairs. Some remain secretive, posing as reclusive hermits, while others keep a high profile, openly ruling factions. Tian cultures in turn venerate the dragons, depicting gods in the form of dragons or claiming ancestry from them. Not all are adored, for plenty of these creatures act wickedly. Draconic Cycles Draconic Cycles Five elements underpin the magical powers of imperial dragons, influencing their relationships to all things and, especially, to others of their kind. These elements interlink in two cycles. In the first cycle, each element feeds one other wood feeds fire, fire feeds earth, earth feeds metal, metal feeds water, and water feeds wood. In the second cycle, each element counters another wood counters earth, earth counters water, water counters fire, fire counters metal, and metal counters wood. Each imperial dragon represents one element and has four abilities related to the cycle. For example, the forest dragon—linked to wood—feeds fire, is fed by water, counters earth, and is countered by metal. Shape-Changing Dragons Shape-Changing Dragons Imperial dragons are the most likely (and willing) of Golarion's dragons to take humanoid forms. The vast majority of them have the following ability, with the tradition trait matching the dragon's innate spells 変身 [one-action] (精神集中、完全変身、変成術) The dragon takes on the appearance of any Small or Medium humanoid. This doesn't change their Speed or attack and damage modifiers with their Strikes, but it might change the damage type their Strikes deal (typically to bludgeoning). Imperial Dragon Spellcasters Imperial Dragon Spellcasters Each imperial dragon has a sidebar on spellcasting dragons of that kind. To make an imperial dragon spellcaster, remove the dragon's Coiling Frenzy and Draconic Momentum abilities and give them the spells outlined in their sidebar. You can swap any number of these spells with others from the same tradition, provided you keep the same number of spells for each level. You might also want to increase the dragon's Intelligence, Wisdom, or Charisma modifier by 1 or 2 to reflect their mastery of magic. Imperial Dragons On Golarion Imperial Dragons On Golarion Imperial dragons are rarely spotted beyond Tian Xia, where some establish territories to amass power, while others follow their natural affinity to seek a place of comfort. 関連する種 アンダーワールド・ドラゴン、シー・ドラゴン、、ソヴリン・ドラゴン、フォレスト・ドラゴン
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diom /// / こめかみ \ 14 seren klel sid dzihomain物をかむと動く部分の意 \ [ ova ] \ diom it ma fir こめかみが引きつる \
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【登録タグ Alstroemeria RecordsCD CD CDL】 サークル：Alstroemeria Records 01 Civilization of Magic 02 Complete Darkness 03 Strawberry Crisis!! 04 Inanimate Dream 05 Infinite Being 06 Septet pour une Infante defunte 07 Border of Life 08 Voyage 1970 09 Fate of Sixty Years 10 風神少女 11 風神少女(OFF VOCAL)
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Pax Biologiaとは Pax Biologiaは、埼玉大学に平成21年度、新たに設立されたサークルです。 当サークルは、生物学への興味を増進し、理解を深めることを目的としています。
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Subjects-Engineering-Civil-Environmental-General Subjects-Engineering-Civil-Environmental-Pollution-Air? Subjects-Nonfiction-CurrentEvents-Poverty-SocialServices&Welfare? Subjects-Outdoors&Nature-Environment-EnvironmentalScience Subjects-Professional&Technical-Engineering-Civil-Environmental-General Subjects-Professional&Technical-Engineering-Civil-Environmental-Pollution-Air? Subjects-Professional&Technical-Engineering-SpecialTopics-AppliedAtmosphericSciences? Subjects-Professional&Technical-ProfessionalScience-EarthSciences-EnvironmentalScience? Subjects-Science-EarthSciences-EnvironmentalScience Subjects-Science-General Air Pollution Modeling and Its Application II (Nato Challenges of Modern Society) Plenum Pub Corp? Netherlands) International Technical Meeting on Air Pollution Modeling and Its Application (11th 1980 Amsterdam? C. De Wispelaere? North Atlantic Treaty Organization. Committee on the Challenges of Modern Society? C. De De Wispelaere? PlenumPubCorp? Netherlands)InternationalTechnicalMeetingonAirPollutionModelingandItsApplication(11th 1980 Amsterdam? C.DeWispelaere? NorthAtlanticTreatyOrganization.CommitteeontheChallengesofModernSociety? C.DeDeWispelaere? ジャンル別? Subjects-Engineering-Civil-Environmental-General Subjects-Engineering-Civil-Environmental-Pollution-Air? Subjects-Engineering-General? Subjects-Professional&Technical-Engineering-Civil-Environmental-General Subjects-Professional&Technical-Engineering-Civil-Environmental-Pollution-Air? Subjects-Professional&Technical-Engineering-General Subjects-Professional&Technical-ProfessionalScience-Physics-General? Subjects-Science-General Subjects-Science-Physics-General? Beneficial Effects of Endotoxins Plenum Pub Corp? Alois Nowotny? PlenumPubCorp? AloisNowotny? ジャンル別? Substores-UnknownASINs-7? Subjects-Engineering-Civil-Environmental-General Subjects-Medicine-BasicScience-Physiology? Subjects-Medicine-Pharmacology-General? 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Subjects-Engineering-Civil-Environmental-General Subjects-Engineering-Civil-Environmental-Pollution-Air? Subjects-Engineering-General? Subjects-Professional&Technical-Engineering-Civil-Environmental-General Subjects-Professional&Technical-Engineering-Civil-Environmental-Pollution-Air? Subjects-Professional&Technical-Engineering-SpecialTopics-AppliedAtmosphericSciences? Subjects-Professional&Technical-Engineering-General Subjects-Professional&Technical-ProfessionalScience-Physics-General? Subjects-Science-General Subjects-Science-Physics-General? Subjects-Science-Astronomy-Comets,Meteors&Asteroids? Mass Spectrometry in Environmental Sciences Plenum Pub Corp? F. W. Karasek? O. Hutzinger? S. Safe? PlenumPubCorp? F.W.Karasek? O.Hutzinger? S.Safe? ジャンル別? Substores-UnknownASINs-7? Subjects-Engineering-Civil-Environmental-General Subjects-Engineering-General? Subjects-Outdoors&Nature-Ecology-General? 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Subjects-Science-General Subjects-Science-Physics-General? Subjects-Science-Astronomy-Comets,Meteors&Asteroids? Appropriate Waste Management for Developing Countries Plenum Pub Corp? Kriton Curi? PlenumPubCorp? KritonCuri? ジャンル別? Subjects-Engineering-Civil-Environmental-General Subjects-Engineering-Civil-Environmental-SolidWasteManagement? Subjects-Nonfiction-CurrentEvents-Poverty-SocialServices&Welfare? Subjects-Outdoors&Nature-Environment-Recycling? Subjects-Professional&Technical-Engineering-Civil-Environmental-General Subjects-Professional&Technical-Engineering-Civil-Environmental-SolidWasteManagement? Subjects-Professional&Technical-Medical-BasicSciences-Biology? Subjects-Science-General Subjects-Science-Nature&Ecology-Environment-Recycling? Subjects-Science-Technology-General&Reference Subjects-Science-BiologicalSciences-Biology-General? Carbon-Nitrogen-Sulfur Human Interference in Grand Biospheric Cycles (Modern Perspectives in Energy) Plenum Pub Corp? Vaclav Smil? PlenumPubCorp? VaclavSmil? ジャンル別? Substores-UnknownASINs-7? Subjects-Engineering-Civil-Environmental-General Subjects-Engineering-Energy-General? Subjects-Nonfiction-CurrentEvents-Poverty-SocialServices&Welfare? Subjects-Outdoors&Nature-Environment-EnvironmentalScience Subjects-Professional&Technical-Engineering-Civil-Environmental-General Subjects-Professional&Technical-Engineering-Energy-General? Subjects-Professional&Technical-ProfessionalScience-EarthSciences-EnvironmentalScience? Subjects-Science-Chemistry-Industrial&Technical? Subjects-Science-EarthSciences-EnvironmentalScience Subjects-Science-Experiments,Instruments&Measurement-ElectronMicroscopes&Microscopy? Acid Deposition Environmental, Economic, and Policy Issues Plenum Pub Corp? Donald D. Adams? Walter P. Page? PlenumPubCorp? DonaldD.Adams? WalterP.Page? ジャンル別? Subjects-Engineering-Civil-Environmental-General Subjects-Engineering-Civil-Environmental-Pollution-Water? Subjects-Engineering-Civil-Environmental-Pollution-Air? 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Air Pollution Modeling and Its Application V (Air Pollution Modeling and Its Application) Plenum Pub Corp? C. De De Wispelaere? Francis A. Schiermeier? Noor Gillani? PlenumPubCorp? C.DeDeWispelaere? FrancisA.Schiermeier? NoorGillani? ジャンル別? Subjects-Engineering-SpecialTopics-AppliedAtmosphericSciences? Subjects-Engineering-Civil-Environmental-General Subjects-Engineering-Civil-Environmental-Pollution-Air? Subjects-Engineering-General? Subjects-Outdoors&Nature-Environment-EnvironmentalScience Subjects-Professional&Technical-Engineering-Civil-Environmental-General Subjects-Professional&Technical-Engineering-Civil-Environmental-Pollution-Air? Subjects-Professional&Technical-Engineering-SpecialTopics-AppliedAtmosphericSciences? Subjects-Professional&Technical-Engineering-General Subjects-Professional&Technical-ProfessionalScience-EarthSciences-EnvironmentalScience? 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Subjects-Science-General Subjects-Science-Nature&Ecology-General? Environmental Radon (Environmental Science Research) Plenum Pub Corp? C. Richard Cothem? PlenumPubCorp? C.RichardCothem? ジャンル別? Substores-UnknownASINs-7? Subjects-Engineering-Civil-Environmental-General Subjects-Engineering-Civil-Environmental-Pollution-General? Subjects-Engineering-General? Subjects-Medicine-Administration&Policy-General? Subjects-Medicine-Administration&Policy-PublicHealth? Subjects-Outdoors&Nature-Environment-EnvironmentalScience Subjects-Professional&Technical-Engineering-Civil-Environmental-General Subjects-Professional&Technical-Engineering-Civil-Environmental-Pollution-General? Subjects-Professional&Technical-Engineering-General Subjects-Professional&Technical-Medical-Administration&MedicineEconomics-General? Subjects-Professional&Technical-Medical-Administration&MedicineEconomics-PublicHealth-General? 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Molens Pepermunt Snuff Brand De Kralingse Name Pepermunt Style scented Grind med coarse Moisture moist Kick 4 nicotine 4 Recommend 5 Notes(JPN) 刻みは若干粗めで、適度なキック。燻されたLatakia葉の重厚な香りと微かに鼻の奥に残るミントの清涼感。ミントの効かせ具合が絶妙でお薦めできるスナッフ。煙草でいうとゴロワーズ・ジタン系の香り。 Notes(EN) another review shop @marscigars.com Snuff based on the Latakia Ao 1860 and scented with peppermint and eucalyptus oil. bob @Snuffhouse.org I love this one. A favorite for sure. It kind of reminds me of a dark chocolate mint. At least it s general tonal range(I hope that makes sense.) The two problems with this snuff are first that when I ordered it from mars I went through it in a bout two days, the second problem is I find this one hard to stop using I tend to take pinch after pinch (chain snuffing). Wonderfull. BigSnoot McSnuff @Snuffhouse.org Just got this one in yesterday. The peppermint is indeed very strong and stays with you a long time. Closest I can come to describing it is it smells like you have a snoot full of Scope mouthwash. It is somehow refreshing though like brushing your teeth in the morning or taking a hot shower after a long day of work. Not an all day sniffer for me, but most likely a once a day snuff. Lots of respect for DeKralingse, I have yet to try a snuff of theirs I haven t enjoyed. Now if Mars will just get the Orange in stock so I can complete my DeKralingse line.... Just a quick update, I took a couple of big pinches of this snuff again this morning. I think that perhaps my nose has gotten used to the peppermint because this time the Latakia really came through. Completely changed my opinion of this product and now I think this will be going into my rotation of all day snuffs after all. It is very similar to the AP in that aspect. Once you get past the main scent the Latakia goodness hits you just right. Good stuff!
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Apples to Apples 項目数：12 総ポイント：200 難易度： 製品情報：http //marketplace.xbox.com/en-US/Product/Apples-to-Apples/ 配信日：2011年12月7日 DL費用：800MSP ジャンル：カード＆ボード ☆国内未配信 ※配信終了 Apple-opolis Unlock all the Apple Avatars.(全てのApple Avatarをアンロックする。) 30 Rotten to the Core Be targeted by the "Rotten Apple" card 10 times.("Rotten Apple"のカードのターゲットに10回選ばれる。) 30 An Apple A Day Reach the Orchard Rank in Xbox LIVE by playing 500 games.(Xbox LIVEで500ゲームプレイしてOrchard Rankに到達する。) 30 Falling Far from the Tree Play the "Every Apple for Himself" card 5 times.("Every Apple for Himself"のカードを5回使用する。) 20 Apple of My Eye Win your first Xbox LIVE game.(Xbox LIVE gameで初めて勝利する。) 10 Sour Apple Play a Golden Apple Card for the first time.(Golden Apple Cardを初めて使用する。) 5 Mad Apple Lose 3 Xbox LIVE games in a row.(Xbox LIVE gameで3回連続で敗北する。) 10 All Your Apples Are Belong to Us Play the "Apple Tart" card 10 times.("Apple Tart"のカードを10回使用する。) 10 No Apples for You Play the "Yes, We Have No Apples" card 10 times.("Yes, We Have No Apples"のカードを10回使用する。) 20 Apple Seeds Complete the Single Player tutorial.(Single Player tutorialを完了する。) 5 Apple-ocalypse Be the second player to play an "Upset the Apple Cart" in a single round.(1回のround内で"Upset the Apple Cart"のカードを2番目に使用したプレイヤーとなる。) 20 Social Addict Enable Social Integration for posting achievements.(実績獲得の為に社会的統合を可能にする。) 10 Social Addict 近年増えつつあるFacebook経由で解除する実績の模様。 参考(英語) http //www.trueachievements.com/viewcomment.aspx?commentid=387412#vch
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https //llamalab.com/automate/doc/block/calendar_event_query.html Calendar event query decision block that search for, or await calendar events. 即時実行された場合は、現在実施中のイベントが無いか、最小タイムスタンプと最大タイムスタンプの交わるイベントが無いか、fiberを止めることなくチェックする If proceed is set to When transition, then the fiber will pause until an upcoming event has begun and proceed through the YES path, or an ongoing event has ended and proceed through the NO path. The start offset and end offset can be used to match events before (negative offset) or after (positive offset) the time they’re actually starting and/or ending. Use the Calendar event get block to get the details of an event. Input arguments Calendar URI — カレンダーのURI、デフォルトでは表示中のカレンダーすべて。 Minimum timestamp UNIXタイムスタンプ値。 timestamp of the earliest events to matchデフォルトは現在時刻（Immediately の場合） Maximum timestamp — unix timestamp of the latest events to matchデフォルトは現在時刻（Immediately の場合） Start offset 開始時刻に加えるオフセット（秒）default is 0. End offset — offset in seconds, added the event end time when matchingdefault is 0. Title — title of events to match, default is any title, may contain glob pattern. Description — description of events to match, default is any description, may contain glob pattern. Location name — location of events to match,デフォルトはすべての場所 may contain glob pattern. Availability — availabilities of events, default is any availability. All day — 終日のイベントを無視するか？ Output variables Event URIs — variable to assign the URIs of the intersecting or transitioning events.